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Nat Commun. 2024 09 3;15(1):7692

A Time and Cost-Effective Pipeline for Expression Screening and Protein Production in Insect Cells Based on the HR-Bac Toolbox to Generate Recombinant Baculoviruses.

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A lipid transfer protein ensures nematode cuticular impermeability.

Njume FN, Razzauti A, Soler M, Perschin V, Fazeli G, Bourez A, Delporte C, Ghogomu SM, Poelvoorde P, Pichard S, Birck C, Poterszman A, Souopgui J, Van Antwerpen P, Stigloher C, Vanhamme L, Laurent P

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HR-Bac, a toolbox based on homologous recombination for expression, screening and production of multiprotein complexes using the baculovirus expression system.

Kolesnikova O, Zachayus A, Pichard S, Osz J, Rochel N, Rossolillo P, Kolb-Cheynel I, Troffer-Charlier N, Compe E, Bensaude O, Berger I, Poterszman A

Sci Rep. 2022 02 7;12(1):2030

Insect Cells-Baculovirus System for the Production of Difficult to Express Proteins: From Expression Screening for Soluble Constructs to Protein Quality Control.

Pichard S, Troffer-Charlier N, Kolb-Cheynel I, Poussin-Courmontagne P, Abdulrahman W, Birck C, Cura V, Poterszman A

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A Novel Nanobody Precisely Visualizes Phosphorylated Histone H2AX in Living Cancer Cells under Drug-Induced Replication Stress.

Moeglin E, Desplancq D, Stoessel A, Massute C, Ranniger J, McEwen AG, Zeder-Lutz G, Oulad-Abdelghani M, Chiper M, Lafaye P, Di Ventura B, Didier P, Poterszman A, Weiss E

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Community-wide experimental evaluation of the PROSS stability-design method.

Peleg Y, Vincentelli R, Collins BM, Chen KE, Livingstone EK, Weeratunga S, Leneva N, Guo Q, Remans K, Perez K, E K Bjerga G, Larsen Ø, Vaněk O, Skořepa O, Jacquemin S, Poterszman A, Kjaer S, Christodoulou E, Albeck S, Dym O, Ainbinder E, Unger T, Schuetz A, Matthes S, Bader M, de Marco A, Storici P, Semrau MS, Stolt-Bergner P, Aigner C, Suppmann S, Goldenzweig A, Fleishman SJ

J Mol Biol. 2021 Mar 26;:166964

Gene Tagging with the CRISPR-Cas9 System to Facilitate Macromolecular Complex Purification.

Geny S, Pichard S, Poterszman A, Concordet JP

Methods Mol Biol. 2021 ;2305:153-174

Production of Multiprotein Complexes Using the Baculovirus Expression System: Homology-Based and Restriction-Free Cloning Strategies for Construct Design.

Rossolillo P, Kolesnikova O, Essabri K, Zamorano GR, Poterszman A

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Tagging Proteins with Fluorescent Reporters Using the CRISPR/Cas9 System and Double-Stranded DNA Donors.

Geny S, Pichard S, Brion A, Renaud JB, Jacquemin S, Concordet JP, Poterszman A

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In TFIIH the Arch domain of XPD is mechanistically essential for transcription and DNA repair.

Peissert S, Sauer F, Grabarczyk DB, Braun C, Sander G, Poterszman A, Egly JM, Kuper J, Kisker C

Nat Commun. 2020 Apr 3;11(1):1667

Identification and characterization of the Onchocerca volvulus Excretory Secretory Product Ov28CRP, a putative GM2 activator protein.

Njume FN, Ghogomu SM, Shey RA, Gainkam LOT, Poelvoorde P, Humblet P, Kamgno J, Robert A, Mutesa L, Lelubre C, Edelweiss E, Poterszman A, Anheuser S, Vanhamme L, Souopgui J

PLoS Negl Trop Dis. 2019 Jul 22;13(7):e0007591

Functional interplay between TFIIH and KAT2A regulates higher-order chromatin structure and class II gene expression.

Sandoz J, Nagy Z, Catez P, Caliskan G, Geny S, Renaud JB, Concordet JP, Poterszman A, Tora L, Egly JM, Le May N, Coin F

Nat Commun. 2019 Mar 20;10(1):1288

TFIIH: A multi-subunit complex at the cross-roads of transcription and DNA repair.

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Adv Protein Chem Struct Biol. 2019 ;115:21-67

The intricate network between the p34 and p44 subunits is central to the activity of the transcription/DNA repair factor TFIIH.

Radu L, Schoenwetter E, Braun C, Marcoux J, Koelmel W, Schmitt DR, Kuper J, Cianférani S, Egly JM, Poterszman A, Kisker C

Nucleic Acids Res.. 2017 Aug;:

Crystallization and Preliminary X-Ray Diffraction Analysis of a Mammal Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase.

Franco-Echevarría E, Sanz-Aparicio J, Troffer-Charlier N, Poterszman A, González B

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An evolutionary conserved Hexim1 peptide binds to the Cdk9 catalytic site to inhibit P-TEFb.

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Baculovirus Expression: Old Dog, New Tricks.

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Bioengineered. 2015 Oct 21:0.

Insect cells-baculovirus system for the production of difficult to express proteins.

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Methods Mol Biol. 2015;1258:181-205

The production of multiprotein complexes in insect cells using the baculovirus expression system.

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In TFIIH, XPD helicase is exclusively devoted to DNA repair.

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PLoS Biol. 2014 Sep 30;12(9):e1001954

Expression of functional full-length hSRC-1 in eukaryotic cells using modified vaccinia virus Ankara and baculovirus.

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Anal Biochem. 2012 Jul 15;426(2):106-8

Robots, pipelines, polyproteins: enabling multiprotein expression in prokaryotic and eukaryotic cells.

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J Struct Biol. 2011 Aug;175(2):198-208

Structural insights into transcription complexes.

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J Struct Biol. 2011 Aug;175(2):135-46

Analysis of recombinant phosphoprotein complexes with complementary mass spectrometry approaches.

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Anal Biochem. 2010 Dec 1;407(1):34-43

Structure determination of the minimal complex between Tfb5 and Tfb2, two subunits of the yeast transcription/DNA-repair factor TFIIH: a retrospective study.

Kainov DE, Cura V, Vitorino M, Nierengarten H, Poussin P, Kieffer B, Cavarelli J, Poterszman A

Acta Crystallogr D Biol Crystallogr. 2010 Jul;66(Pt 7):745-55

A set of baculovirus transfer vectors for screening of affinity tags and parallel expression strategies.

Abdulrahman W, Uhring M, Kolb-Cheynel I, Garnier JM, Moras D, Rochel N, Busso D, Poterszman A

Anal Biochem. 2009 Feb 15;385(2):383-5

Structural basis for group A trichothiodystrophy

Kainov DE, Vitorino M, Cavarelli J, Poterszman A, Egly JM

Nat Struct Mol Biol. 2008 Sep;15(9):980-4.

Solution structure and self-association properties of the p8 TFIIH subunit responsible for trichothiodystrophy.

Vitorino M, Coin F, Zlobinskaya O, Atkinson RA, Moras D, Egly JM, Poterszman A, Kieffer B

J Mol Biol. 2007 Apr 27;368(2):473-80

[DNA helicases and human diseases].

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Med Sci (Paris). 2006 Dec;22(12):1087-94.

Biophysical characterization of an indolinone inhibitor in the ATP-binding site of DNA gyrase.

Oblak M, Grdadolnik SG, Kotnik M, Poterszman A, Atkinson RA, Nierengarten H, Desplancq D, Moras D, Solmajer T

Biochem Biophys Res Commun. 2006 Nov 3;349(4):1206-13

Co-expression of protein complexes in prokaryotic and eukaryotic hosts: experimental procedures, database tracking and case studies.

Romier C, Ben Jelloul M, Albeck S, Buchwald G, Busso D, Celie PH, Christodoulou E, De Marco V, van Gerwen S, Knipscheer P, Lebbink JH, Notenboom V, Poterszman A, Rochel N, Cohen SX, Unger T, Sussman JL, Moras D, Sixma TK, Perrakis A

Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1232-42

Eukaryotic expression: developments for structural proteomics.

Aricescu AR, Assenberg R, Bill RM, Busso D, Chang VT, Davis SJ, Dubrovsky A, Gustafsson L, Hedfalk K, Heinemann U, Jones IM, Ksiazek D, Lang C, Maskos K, Messerschmidt A, Macieira S, Peleg Y, Perrakis A, Poterszman A, Schneider G, Sixma TK, Sussman JL, Sutton G, Tarboureich N, Zeev-Ben-Mordehai T, Jones EY

Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1114-24

First steps towards effective methods in exploiting high-throughput technologies for the determination of human protein structures of high biomedical value.

Banci L, Bertini I, Cusack S, de Jong RN, Heinemann U, Jones EY, Kozielski F, Maskos K, Messerschmidt A, Owens R, Perrakis A, Poterszman A, Schneider G, Siebold C, Silman I, Sixma T, Stewart-Jones G, Sussman JL, Thierry JC, Moras D

Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1208-17

Structure and oligomeric state of human transcription factor TFIIE.

Jawhari A, Uhring M, De Carlo S, Crucifix C, Tocchini-Valentini G, Moras D, Schultz P, Poterszman A

EMBO Rep. 2006 May;7(5):500-5

Solution structure of the C-terminal domain of TFIIH P44 subunit reveals a novel type of C4C4 ring domain involved in protein-protein interactions.

Kellenberger E, Dominguez C, Fribourg S, Wasielewski E, Moras D, Poterszman A, Boelens R, Kieffer B

J Biol Chem. 2005 May 27;280(21):20785-92

Domain architecture of the p62 subunit from the human transcription/repair factor TFIIH deduced by limited proteolysis and mass spectrometry analysis.

Jawhari A, Boussert S, Lamour V, Atkinson RA, Kieffer B, Poch O, Potier N, van Dorsselaer A, Moras D, Poterszman A

Biochemistry. 2004 Nov 16;43(45):14420-30.