Cytoplasmic overexpression of RNA-binding protein HuR is a marker of poor prognosis in meningioma, and HuR knockdown decreases meningioma cell growth and resistance to hypoxia.
Fiche publication
Date publication
mai 2017
Journal
The Journal of pathology
Auteurs
Membres identifiés du Cancéropôle Est :
Pr GUEANT Jean-Louis, Dr HOULGATTE Rémy, Pr VIGNAUD Jean-Michel, Pr GAUCHOTTE Guillaume, Dr BATTAGLIA-HSU Shyue-Fang, Dr RECH Fabien
Tous les auteurs :
Gauchotte G, Hergalant S, Vigouroux C, Casse JM, Houlgatte R, Kaoma T, Helle D, Brochin L, Rech F, Peyre M, Labrousse F, Vallar L, Gueant JL, Vignaud JM, Battaglia-Hsu SF
Lien Pubmed
Résumé
HuR regulates cytoplasmic mRNA stability and translatability, and the HuR expression level has been shown to correlate with poor disease outcome in several cancer types; however, the prognostic value and potential pro-oncogenic properties of HuR in meningioma remain unclear. Thus, in the present study we analyzed 85 meningioma tissue samples to establish the relationship between HuR expression, tumor-cell proliferation, and/or patient survival. In addition, we examined the anti-proliferative effects of HuR knockdown in two meningioma cell lines (IOMM-Lee and Ben-Men1), and conducted transcriptome-wide analyses (IOMM-Lee cells) to elucidate the molecular consequences of HuR knockdown. The results of the present study showed HuR cytoplasmic expression to correlate positively with tumor grade (P = 1.2 x 10 (-8) ), and negatively with progression-free and overall survival (P = 0.01) time in human meningioma tissues. In vitro, siHuR-induced HuR knockdown was shown to reduce the growth of both Ben-Men-1 (P = 2 x 10(-8) ) and IOMM-Lee (P = 4 x 10(-9) ) cells. Transcriptome analyses revealed HuR knockdown in IOMM-Lee cells to deregulate the HIF1A signaling pathway (P = 1.5 x 10(-6) ), and to upregulate the expression of genes essential for the assembly of the cytoplasmic mRNA processing body, global genome nucleotide-excision repair, poly(A)-specific ribonuclease activity, the positive regulation of apoptosis and of cell cycle arrest, and the negative regulation of RNA splicing (P(FDR) < 0.001). Interestingly, HuR knockdown under hypoxic culture conditions further potentiated the effects of HuR knockdown on cell growth, apoptosis, and HIF1A expression. We thus conclude that cytoplasmic HuR expression is a marker of poor prognosis in meningioma, and that HuR is a promising potential therapeutic target for use in tumors refractory to standard therapies.
Mots clés
ELAV-like 1, HuR, apoptosis, hypoxia, meningioma, proliferation
Référence
J. Pathol.. 2017 May;: