4C-Seq: Interrogating Chromatin Looping with Circular Chromosome Conformation Capture.
Fiche publication
Date publication
janvier 2021
Journal
Methods in molecular biology (Clifton, N.J.)
Auteurs
Membres identifiés du Cancéropôle Est :
Dr SEXTON Thomas
Tous les auteurs :
Karasu N, Sexton T
Lien Pubmed
Résumé
Chromosome conformation capture and its variants have allowed chromatin topology to be interrogated at a superior resolution and throughput than by microscopic methods. Among the method derivatives, 4C-seq (circular chromosome conformation capture, coupled to high-throughput sequencing) is a versatile, cost-effective means of assessing all chromatin interactions with a specific genomic region of interest, making it particularly suitable for interrogating chromatin looping events. We present the principles and procedures for designing and implementing successful 4C-seq experiments.
Mots clés
Chromatin fixation, Chromatin loops, Circular chromosome conformation capture, Genome topology, High-throughput sequencing, Inverse PCR, Ligation, Restriction digestion
Référence
Methods Mol. Biol.. 2021 ;2157:19-34