Design of antibody-functionalized carbon nanotubes filled with radioactivable metals towards a targeted anticancer therapy.
Fiche publication
Date publication
juillet 2016
Journal
Nanoscale
Auteurs
Membres identifiés du Cancéropôle Est :
Dr MENARD-MOYON Cécilia, Dr BIANCO Alberto
Tous les auteurs :
Spinato C, Perez Ruiz de Garibay A, Kierkowicz M, Pach E, Martincic M, Klippstein R, Bourgognon M, Wang JT, Ménard-Moyon C, Al-Jamal KT, Ballesteros B, Tobias G, Bianco A
Lien Pubmed
Résumé
In the present work we have devised the synthesis of a novel promising carbon nanotube carrier for the targeted delivery of radioactivity, through a combination of endohedral and exohedral functionalization. Steam-purified single-walled carbon nanotubes (SWCNTs) have been initially filled with radioactive analogues (i.e. metal halides) and sealed by high temperature treatment, affording closed-ended CNTs with the filling material confined in the inner cavity. The external functionalization of these filled CNTs was then achieved by nitrene cycloaddition and followed by the derivatization with a monoclonal antibody (Cetuximab) targeting the epidermal growth factor receptor (EGFR), overexpressed by several cancer cells. The targeting efficiency of the so-obtained conjugate was evaluated by immunostaining with a secondary antibody and by incubation of the CNTs with EGFR positive cells (U87-EGFR+), followed by flow cytometry, confocal microscopy or elemental analyses. We demonstrated that our filled and functionalized CNTs can internalize more efficiently in EGFR positive cancer cells.
Mots clés
Animals, CHO Cells, Cell Line, Tumor, Cetuximab, administration & dosage, Cricetulus, ErbB Receptors, Humans, Metals, administration & dosage, Nanotubes, Carbon, Neoplasms, radiotherapy, Radiotherapy
Référence
Nanoscale. 2016 Jul 7;8(25):12626-38