DHA induces Jurkat T-cell arrest in G2/M phase of cell cycle and modulates the plasma membrane expression of TRPC3/6 channels.
Fiche publication
Date publication
décembre 2020
Journal
Biochimie
Auteurs
Membres identifiés du Cancéropôle Est :
Pr KAHN Naim, Dr HICHAMI Aziz
Tous les auteurs :
Saidi H, Murtaza B, Khan AS, Koceir EA, Hichami A, Khan NA
Lien Pubmed
Résumé
We investigated whether docosahexaenoic acid (DHA), a dietary n-3 fatty acid, modulates calcium (Ca) signaling and cell cycle progression in human Jurkat T-cells. Our study demonstrates that DHA inhibited Jurkat T-cell cycle progression by blocking their passage from S phase to G2/M phase. In addition, DHA decreased the plasma membrane expression of TRPC3 and TRPC6 calcium channels during T-cell proliferation. Interestingly, this fatty acid increased plasma membrane expression of TRPC6 after 24 h of mitogenic stimulation by phorbol-13-myristate-12-acetate (PMA) and ionomycin. These variations in the membrane expression of TRPC3 and TRPC6 channels were not directly correlated with the mRNA expression, indicating that it was a post-translational phenomenon. DHA increased free intracellular calcium concentrations, [Ca]i, via opening TRPC3 and TRPC6 channels. We conclude that the anti-proliferative effect of DHA might involve the modulation of TRPC3 and TRPC6 channels in human T-cells.
Mots clés
Cell cycle, DHA, Jurkat T-cells, TRPC3, TRPC6
Référence
Biochimie. 2020 Dec 14;: