Intasome architecture and chromatin density modulate retroviral integration into nucleosome.

Fiche publication


Date publication

février 2015

Journal

Retrovirology

Auteurs

Membres identifiés du Cancéropôle Est :
Dr RUFF Marc


Tous les auteurs :
Benleulmi MS, Matysiak J, Henriquez DR, Vaillant C, Lesbats P, Calmels C, Naughtin M, Leon O, Skalka AM, Ruff M, Lavigne M, Andreola ML, Parissi V

Résumé

Retroviral integration depends on the interaction between intasomes, host chromatin and cellular targeting cofactors as LEDGF/p75 or BET proteins. Previous studies indicated that the retroviral integrase, by itself, may play a role in the local integration site selection within nucleosomal target DNA. We focused our study on this local association by analyzing the intrinsic properties of various retroviral intasomes to functionally accommodate different chromatin structures in the lack of other cofactors.

Mots clés

Chromatin, metabolism, DNA, metabolism, Host-Pathogen Interactions, Humans, Integrases, metabolism, Nucleosomes, metabolism, Retroviridae, physiology, Virus Integration

Référence

Retrovirology. 2015 Feb;12:13