An atlas of the human liver diurnal transcriptome and its perturbation by hepatitis C virus infection.
Fiche publication
Date publication
août 2024
Journal
Nature communications
Auteurs
Membres identifiés du Cancéropôle Est :
Pr BAUMERT Thomas, Dr DAVIDSON Irwin, Dr LUPBERGER Joachim, Dr MUKHERJI Atish, Dr CROUCHET Emilie, Dr BALTZINGER Philippe
Tous les auteurs :
Mukherji A, Jühling F, Simanjuntak Y, Crouchet E, Del Zompo F, Teraoka Y, Haller A, Baltzinger P, Paritala S, Rasha F, Fujiwara N, Gadenne C, Slovic N, Oudot MA, Durand SC, Ponsolles C, Schuster C, Zhuang X, Holmes J, Yeh ML, Abe-Chayama H, Heikenwälder M, Sangiovanni A, Iavarone M, Colombo M, Foung SKH, McKeating JA, Davidson I, Yu ML, Chung RT, Hoshida Y, Chayama K, Lupberger J, Baumert TF
Lien Pubmed
Résumé
Chronic liver disease and cancer are global health challenges. The role of the circadian clock as a regulator of liver physiology and disease is well established in rodents, however, the identity and epigenetic regulation of rhythmically expressed genes in human disease is less well studied. Here we unravel the rhythmic transcriptome and epigenome of human hepatocytes using male human liver chimeric mice. We identify a large number of rhythmically expressed protein coding genes in human hepatocytes of male chimeric mice, which includes key transcription factors, chromatin modifiers, and critical enzymes. We show that hepatitis C virus (HCV) infection, a major cause of liver disease and cancer, perturbs the transcriptome by altering the rhythmicity of the expression of more than 1000 genes, and affects the epigenome, leading to an activation of critical pathways mediating metabolic alterations, fibrosis, and cancer. HCV-perturbed rhythmic pathways remain dysregulated in patients with advanced liver disease. Collectively, these data support a role for virus-induced perturbation of the hepatic rhythmic transcriptome and pathways in cancer development and may provide opportunities for cancer prevention and biomarkers to predict HCC risk.
Mots clés
Humans, Liver, metabolism, Animals, Transcriptome, Male, Hepatocytes, metabolism, Mice, Hepacivirus, genetics, Hepatitis C, genetics, Circadian Rhythm, genetics, Liver Neoplasms, genetics, Circadian Clocks, genetics, Epigenesis, Genetic
Référence
Nat Commun. 2024 08 29;15(1):7486